For the cross-compartment analysis, only cell clusters with with more than 100 cells were considered:

Isoform switches

  • Number of isoform-switching genesfound per compartment-comparison (left)
  • Volcano plot showing adjusted p_val and average log fold change per gene (right)

Breakdown of isoform switches per cell type:

Top 10 switching genes

–>

CENPM

RPL14

RTN4

S100A16

MYL6

RPS24

PSMB10

ATP5F1C

ADD3

LIMS1

All isoform switching genes

---
title: "Results of isoform-switch search accross compartments"
output:
  html_notebook:
    css: "style.css"
    code_folding: none
    theme: journal
    toc: yes
    toc_depth: 3
    toc_float: yes
  html_document:
    df_print: paged
    toc: yes
    toc_depth: '3'
  pdf_document:
    toc: yes
    toc_depth: '3'
---


```{r setup, include=FALSE}
knitr::opts_chunk$set(
  echo = FALSE,
  message = FALSE,
  warning = FALSE, 
  cache = FALSE,
  cache.lazy = FALSE,
  tidy = TRUE
)


setwd("/data/10x_data/10x_5psanger")
source("./isoswitch/00.common.import.R")
source("./isoswitch/00.visual.R")
library(treemapify)
```

    
 
```{r }
sanger <- if(exists('sanger')) sanger else readRDS("output/A04-sangerMULTI.rds")
MIN_CELLS_PER_COMPARTMENT = 100 

# subset for cell_types with more than 100 cells
CX_idents <- count_df %>%
 filter(n > MIN_CELLS_PER_COMPARTMENT) %>%
 arrange(desc(n))

Idents(sanger) <- "new_celltype"
sanger.CX <- subset(sanger, idents = CX_idents$new_celltype) 

```


```{r}

cluster_labels = c(
  "Fibroblast\n(C0)",
  "Epithelial\n(C1)",
  "Myeloid\n(C2)",
  "Endothelial\n(C3)",
  "T+NK+ILC\n(C4)",
  "B\n(C5)",
  "Ciliated\n(C6)",
  "PNS\n(C7)",
  "Mast\n(C8)",
  "Hepatoblast\n(C9)"
)

# label compartments for plotting
sanger[['big_cluster_labels']] <- sanger[['big_cluster']]
levels(sanger@meta.data$big_cluster_labels) <- cluster_labels

submat <- as.matrix(sanger@meta.data[c("X_umap_x","X_umap_y")])
colnames(submat) <- c("UMAP_1","UMAP_2")
sanger@reductions$umap@cell.embeddings <- submat

dp <- DimPlot(sanger, reduction = "umap", group.by="big_cluster_labels", label = TRUE, label.size=3.5, pt.size=0.1) + 
  labs(title=NULL) + 
  coord_cartesian(clip = 'off') + 
  theme_void() + 
  theme(legend.position="none") + 
  scale_color_discrete(labels=cluster_labels) + 
  labs(title="Dataset compartments")
dp

```



For the cross-compartment analysis, only cell clusters with with more than 100 cells were considered:


```{r fig.height=5, fig.width=12}

umap_theme = theme(
  axis.ticks = element_blank(),
  axis.text = element_blank(),
  axis.line = element_line(colour = 'gray', size = 0.2),
  axis.title.x = element_text(size=10),
  plot.title = element_text(size=14, face="plain", hjust = 0))

(DimPlot(sanger.CX, group.by="new_celltype", label=TRUE, repel=TRUE, pt.size=0.8, label.size=3.2, raster=TRUE) + 
  labs(y=NULL, x="UMAP", title="Cell type") + 
  coord_cartesian(clip = 'off') +
  umap_theme +
  NoLegend()) | 
(DimPlot(sanger.CX, group.by="stage",shuffle=TRUE, raster=TRUE) +
  labs(y=NULL, x="UMAP", title="Developmental stage") + 
  umap_theme)

```


```{r fig.height=4, fig.width=5}
ggplot(CX_idents, aes(area = n, label = glue("{new_celltype}\n({n})"), subgroup = big_cluster)) +
  geom_treemap( fill="#F8766D" ) +
  geom_treemap_subgroup_border() +
  geom_treemap_subgroup_text(place = "centre", grow=F, alpha = 0.5, colour = "black",
                             fontface = "italic", min.size = 0) +
  geom_treemap_text(colour = "white", place = "topleft", size=10, grow=F, reflow = T) +
  ggtitle(glue("n={sum(CX_idents$n)}"))
```


# Isoform switches

```{r include=FALSE}
DefaultAssay(sanger.CX) <- "multi"
Idents(sanger.CX) <- "new_celltype"
clusters <- levels(sanger.CX@active.ident)

#CX_markers  <- ISO_SWITCH_ALL(sanger.CX, clusters, assay="multi", 
#                             min.pct=0.10, logfc.threshold=1, verbose=TRUE)
#write.table(CX_markers, "./output/A06-CX_markers_logfc1.csv", sep=",")

CX_markers <- read.table("./output/A06-CX_markers_logfc1.csv", sep=",")
CX_switches <- compute_switches(CX_markers)
```


```{r}
df <- CX_switches %>% 
  mutate(cluster1 = str_sub(c1, -2, -1), cluster2 = str_sub(c2, -2, -1)) %>%
  mutate(cluster1 = if_else(cluster1 >= cluster2, cluster1, cluster2)) %>%
  mutate(cluster2 = if_else(cluster1 >= cluster2, cluster2, cluster1)) %>%
  select(cluster1, cluster2, geneId) %>%
  group_by(cluster1, cluster2) %>% 
  distinct(geneId) %>%
  summarise(n=n())
df

clusters = c("C0", "C1","C2", "C3","C4", "C5", "C6")

tmp <- data.frame()
for(i in clusters) {
  for(j in clusters) {
    if(j > i) {
      n <- if(nrow(df[df$cluster1==i & df$cluster2==j, 'n'])==0) 0 else df[df$cluster1==i & df$cluster2==j, 'n'][[1]]
      m <- if(nrow(df[df$cluster1==j & df$cluster2==i, 'n'])==0) 0 else df[df$cluster1==j & df$cluster2==i, 'n'][[1]]
      df[df$cluster1==i & df$cluster2==j, 'n'] <- 0
      df[df$cluster1==j & df$cluster2==i, 'n'] <- 0
      if(t > 0 ) {
        row <- data.frame(i=i, j=j, t= n+m)
        tmp <- rbind(tmp, row)
      }
    }
  }
}

mat_clusters <- 
  ggplot(tmp, aes(x=i, y=j, fill=t)) + 
    geom_tile() +
    geom_text(aes(label=t), size=2) +
    theme_minimal() + 
    scale_fill_distiller(palette="YlOrRd", direction=1) +
    theme(axis.text.x = element_text(angle=45, hjust=1, size=8),
          axis.text.y = element_text(size=8)) + 
    labs(x=NULL, y=NULL) + 
    NoLegend()


#pw <- mat_clusters | dp1 
#pw
#ggsave("img/Fig10_top2_3.png", plot=pw, device="png", width=8, height=3, units="in", dpi=300, type="cairo")


```
* Number of isoform-switching genesfound per compartment-comparison (left)
* Volcano plot showing adjusted p_val and average log fold change per gene (right)

```{r fig.width=12, fig.height=4}

 mat_clusters | plot_marker_score(sanger.CX,CX_markers, facet=FALSE)

# pw <- mat_clusters | dp1 
# ggsave("img/Fig10_top.png", plot=pw, device="png", width=8, height=4, units="in", dpi=300, type="cairo")
```

Breakdown of isoform switches per cell type:
```{r fig.width=13, fig.height=7}
dp <- plot_marker_score(sanger.CX,CX_markers, facet=TRUE, ncol=5)
dp
```



# Top 10 switching genes {.tabset}


```{r gene_list, echo=FALSE}
gene_list <- CX_markers %>%
  group_by(geneId) %>%
  slice_min(order_by=p_val_adj, n=1, with_ties=FALSE) %>%
  arrange(p_val_adj) %>%
  pull(geneId)
```


<!-- only seems to work in reports (??) -  do  manual list instead --> -->

<!-- ```{r fig.height=7, fig.width=13, results='asis'} -->
<!-- {r gene_reports, fig.height=7, fig.width=13, results='asis'} -->
<!-- loop_end = min(10, length(gene_list)) -->
<!-- for(gene in gene_list[1:loop_end]){ -->
<!--   cat("\n\n## ", gene, "\n\n") -->
<!--   p<-isoswitch_report(sanger.CX, "multi", gene, marker_list=CX_markers) -->
<!--   print(p)   -->
<!--   cat("\n\n") -->
<!-- } -->



## CENPM
```{r fig.height=7, fig.width=13}
  print(isoswitch_report(sanger.CX, "multi", "CENPM", marker_list=CX_markers))
```

## RPL14
```{r fig.height=7, fig.width=13}
  print(isoswitch_report(sanger.CX, "multi", "RPL14", marker_list=CX_markers))
```

## RTN4
```{r fig.height=7, fig.width=13}
  print(isoswitch_report(sanger.CX, "multi", "RTN4", marker_list=CX_markers))
```

## S100A16
```{r fig.height=7, fig.width=13}
  print(isoswitch_report(sanger.CX, "multi", "S100A16", marker_list=CX_markers))
```

## MYL6
```{r fig.height=7, fig.width=13}
  print(isoswitch_report(sanger.CX, "multi", "MYL6", marker_list=CX_markers))
```

## RPS24
```{r fig.height=7, fig.width=13}
  print(isoswitch_report(sanger.CX, "multi", "RPS24", marker_list=CX_markers))
```

## PSMB10
```{r fig.height=7, fig.width=13}
  print(isoswitch_report(sanger.CX, "multi", "PSMB10", marker_list=CX_markers))
```

## ATP5F1C
```{r fig.height=7, fig.width=13}
  print(isoswitch_report(sanger.CX, "multi", "ATP5F1C", marker_list=CX_markers))
```

## ADD3
```{r fig.height=7, fig.width=13}
  print(isoswitch_report(sanger.CX, "multi", "ADD3", marker_list=CX_markers))
```


## LIMS1
```{r fig.height=7, fig.width=13}
  print(isoswitch_report(sanger.CX, "multi", "LIMS1", marker_list=CX_markers))
```


```{r generate_gene_reports, include=FALSE}
#length(gene_list)
#for(gene in gene_list) {
#    try(render_html_gene_report(sanger.CX, CX_markers, gene, prefix="CX"))
#}
```


# All isoform switching genes

```{r gene_table}
gene_switch_table(CX_markers, "CX")
```
